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Adeno-Associated Virus-DJ (AAV-DJ) is a synthetic serotype made from eight different wild-type AAV serotypes (AAV2, 4, 5, 8, 9, avian, bovine, and goat AAV) using DNA shuffling. These modifications give the AAV-DJ serotype improved transduction
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Image Search Results
Journal: Nature methods
Article Title: Fast, in vivo voltage imaging using a red fluorescent indicator
doi: 10.1038/s41592-018-0188-7
Figure Lengend Snippet: a, Confocal images of a slice co-electroporated with CheRiff and VARNAM (n = 5 fields of view). Single and double arrows in the merged image indicate neurons expressing one or both constructs, respectively. b,c, Optical and electrical spikes induced by 10 Hz (b) and 50 Hz (c) blue-light pulses (vertical blue lines) (0.5 ms, 0.5–1 mW mm−2). Arrow in c indicates spike failure. Gray box indicates interval shown at an expanded time scale. *Blue-light artifact (1 mW mm−2). d, Fluorescence responses to 400 ms blue-light illumination (blue steps) at increasing intensities (0.4–2.4 mW mm−2). e, Confocal images of a fixed slice from PV-Cre+ mouse showing cell-type-specific VARNAM and CheRiff expression (n = 7 fields-of-view). f,g, Mean optical and electrical IPSPs evoked by (f) blue-light pulses (2.5 mW mm−2) at depolarized (left) and resting potentials (right) and (g) longer trains of pulses at depolarized potentials. n = 15 trials, each. Shading denotes s.e.m.
Article Snippet: We generated a recombinant AAV encoding floxed
Techniques: Expressing, Construct, Fluorescence